2 deoxyuridine edu  (Thermo Fisher)

Journal: Frontiers in Immunology

Article Title: A palmitoylation-associated three-gene signature predicts prognosis, immune features, and drug sensitivity in gastric cancer: functional validation of CTHRC1

doi: 10.3389/fimmu.2026.1893438

Figure Lengend Snippet: Cellular expression and functional validation of CTHRC1 in vitro . (A–C) Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis of SERPINE1, CTHRC1, and EGR1 in GES-1, AGS, HGC-27, and MKN-45 cells. (D) qRT-PCR screening of three CTHRC1-targeting short hairpin RNAs (shRNAs) in HGC-27 and AGS cells. (E) Western blot validation of CTHRC1 knockdown; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was the loading control. (F, G) Cell Counting Kit-8 (CCK-8) proliferation assays. (H, I) Representative 5-ethynyl-2′-deoxyuridine (EdU) staining and quantification; scale bar, 100 µm. (J, K) Colony-formation assays and quantification; scale bar, 10 mm. (L–N) Wound-healing assays and migration-rate quantification; scale bar, 100 µm. (O–R) Transwell migration/invasion assays and quantification; scale bar, 100 µm. Data are mean ± standard deviation (SD) from three independent biological experiments. (A–D) were analyzed by one-way analysis of variance (ANOVA) with Dunnett correction, panels F and G by two-way ANOVA with multiple-comparison correction, and two-group endpoint comparisons by two-sided Student’s t-tests. ***P < 0.001.

Article Snippet: DNA synthesis was assessed using a BeyoClick 5-ethynyl-2′-deoxyuridine (EdU)-594 Cell Proliferation Kit (Beyotime, C0078S).

Techniques: Expressing, Functional Assay, Biomarker Discovery, In Vitro, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Analysis, Screening, Western Blot, Knockdown, Control, Cell Counting, CCK-8 Assay, Staining, Colony Assay, Migration, Standard Deviation, Comparison

Journal: Osteoarthritis and Cartilage Open

Article Title: Arthroscopically and manually minced cartilage demonstrates lower cell viability and lower proteoglycan deposition compared to isolated chondrons and chondrocytes

doi: 10.1016/j.ocarto.2026.100843

Figure Lengend Snippet: Proliferation of minced cartilage and isolated cells on day 28. A biopsy from a chondral allograft, minced cartilage minced manually or arthroscopically and isolated chondrons and chondrocytes were cultured in fibrin gel and proliferation was determined on day 28. (a) Graph showing the percentage of proliferated cells in minced cartilage and isolated cells on day 28. Each point represents a donor with a minimum of one replicate per donor. Each donor is represented by a different symbol. Standard deviations are shown. A GEE analysis with a Bonferroni post-hoc correction for multiple testing between all groups was performed. All statistical significance for comparisons between groups including specific p-values and 95%-CI can be found in the appendix. Statistical differences are shown and marked as following: The chondrocytes group was statistically significantly higher than the arthroscopically and manually minced group. Picture b through f shows proliferated cells (green) and all cells (blue). (b) Chondral allograft biopsy; (c) Manually minced cartilage; (d) Arthroscopically minced cartilage; (e) Chondrons; (f) Chondrocytes. Isolated cell groups show a homogenous distribution of proliferated cells. Minced cartilage groups show less proliferation on the edges in comparison to the chondral allograft biopsy. All pictures taken at 20x magnification.

Article Snippet: To assess cell proliferation, samples were cultured with 5-ethynyl-2′-deoxyuridine (EdU, Invitrogen, Carlsbad, CA, USA).

Techniques: Isolation, Cell Culture, Comparison